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Test Code KLI KLEIHAUER TEST, BLOOD

Method

Acid Elution of Adult Hemoglobin

CPT(s)

Description CPT Code
Kleihauer Test, Blood 85460

Specimen Information

Container

Specimen

Temperature

Collect Vol

Submit Vol

Minimum Vol

Lavender Top Tube Whole Blood

Refrigerate

2.5 mL 2.5 mL 1.5 mL

 

Reference Range

No fetal cells seen

Instrumentation

Manual Method

LOINC Code Information

Result Code Reporting Name LOINC Code
KLI Kleihauer test, Blood 48555-7

 

Performing Location

University of Vermont Medical Center

Test Schedule / Analytical Time / Test Priority

Monday – Friday 7 am to 3:30 only / 1 day / Not available STAT

Clinical Indications

Definitely Indicated

Clinical Judgment

Not Indicated

RhIG (if positive fetal screen)

Maternal Trauma

External cephalic version

Fetal loss > 20wks

Neonatal Anemia

Non-traumatic abruption

Fetal hydrops

Cord blood sample

Vaginal bleeding

High clinical concern for massive fetal maternal hemorrhage with MCA dopplers

 

If the patient is Rh (-) a Fetal Screen Test should be initially ordered.  A Kleihauer-Betke will automatically be reflexed and run on all positive fetal screens.

The Kleihauer-Betke (KB) test is designed to quantitate fetal red blood cells, but the results have been shown to be imprecise with a large coefficient of variation (CV).  It is often utilized to quantitate large fetomaternal hemorrhages at the time of delivery in Rh (-) women for appropriate dosing of RhIG to prevent Rh alloimmunization. Although it can be used during pregnancy in both Rh (-) and Rh (+) women to detect fetomaternal hemorrhage, the evidence to support its utility are limited and results should be interpreted with caution.

 

Section

Hematology

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

Yes