Test Code 8888 Culture (Urine)
Purpose
Identification of pathogens in urine.
Performing Laboratory
Copley Hospital
Methodology
Conventional Culture Technique
Specimen Requirements
Specimen Type: Catheter (Indwelling)
Collection Container/Tube: Syringe
Submission Container/Tube: Sterile Plastic Container
Specimen Volume: 10 mL
Specimen Minimum Volume: 5 mL
Collection Instructions:
1. Avoid sending urine that has remained stagnant in catheter tubing for
any length of time; do not send catheter bag urine; and avoid sending
urine from catheters that have been in place longer than 5 days.
2. Clean catheter collection port with 70% alcohol.
3. Puncture with sterile needle and collect 5 mL to 10 mL of urine.
4. Transfer > 5 mL of urine into sterile container. Mix well.
5. Label specimen with patient’s full name, date of birth,
date & time of collection, and person collecting.
6. Keep specimen refrigerated during transport.
Specimen Type: Catheter (Straight)
Container/Tube: Sterile Plastic Container
Specimen Volume: 10 mL
Specimen Minimum Volume: 5 mL
Collection Instructions:
1. Thoroughly cleanse urethral area with soap and water.
2. Rinse with gauze pads.
3. Aseptically insert catheter into bladder.
4. Allow approximately 15 mL to pass; then collect
10 mL of urine in sterile container. Mix well.
5. Label specimen with patient’s full name, date of birth,
date & time of collection, and person collecting.
6. Keep specimen refrigerated during transport.
Additional Information:
This collection is not recommended for routine urine culture
because of potential contamination problems. Procedure may
introduce urethral flora into bladder.
Specimen Type: Clean Catch
Container/Tube: Sterile Plastic Container
Specimen Volume: 10 mL
Specimen Minimum Volume: 5 mL
Collection Instructions:
1. Refer to "Sterile Mid-Stream Urine" instructions one the left.
2. Label specimen with patient’s full name, date of birth,
date & time of collection, and person collecting.
3. Keep specimen refrigerated during transport.
Reference Values
Normal: None Detected
Critical: Pathogen Detected
Pathogens: Enterobacteriaceae
Pseudomonas Sp. (& other gram negative rods)
Staph. Coagulase-Positive
Staph. Coagulase-Negative
(If female in childbearing years R/O Staph sapro)
Beta-Hemolytic Streptococci
Enterococcus
Yeast
Stability
Refrigerated: 24 Hours
Day(s) Performed
Daily
Analytical Time: 2 Days
Aliases
Urine
Culture
Test Classification & CPT Coding
87088