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HelpTest Code 8500 Coombs Direct (DAT)
Purpose
Demonstrating in vivo coating of red blood cells with IgG or
the complement component C3d in the following settings:
-Autoimmune Hemolytic Anemia
-Hemolytic Transfusion Reactions
-Drug-Induced Hemolytic Anemia
Performing Laboratory
Copley Hospital
Methodology
Hemagglutination
Specimen Requirements
Specimen Type: Whole Blood
Container/Tube: Lavender Top
Specimen Volume: Full Tube
Specimen Minimum Volume: 2 mL
Collection Instructions:
1. Invert tube 8 times immediately after collection.
2. Label specimen with patient’s full name, date of birth,
date & time of collection, and person collecting.
3. Refrigerate specimen during transport.
Reference Values
Normal: Negative
Critical: Positive
Stability
Refrigerated: 2 Days
Day(s) Performed
Daily
Available STAT
Analytical Time: 1 Day
Aliases
Coombs Direct
Direct Coombs
Coombs
Direct Antiglobulin Test
Neonatal Blood Evaluation
Direct Antibody Test
DAT
Test Classification & CPT Coding
86880